Abstarct: Abstract Anthurium (Anthurium andraeanum) is one of the most important ornamental plants in the world. The propagation of anthurium using the traditional methods, propagation by seed, and traditional vegetative, is time-consuming and inefficient on a large scale, and due to the heterozygous result, its marketability quality decreases. In this study, the micropropagation conditions of anthurium were investigated using the optimization of its tissue culture protocols. In this study, Murashige and Skoog (MS) culture medium as well as modified MS medium, and plant growth regulators such as BAP, NAA and 2,4-D at different concentration levels were evaluated. Traits such as determining the best sterilization method, induction of callus formation and somatic embryogenesis, direct regeneration, and rooting rate were investigated. Besides, the effect of ammonium nitrate on shoot regeneration, the effect of sucrose concentration on embryogenesis and callus formation, the effect of leaf explant type on time of callus induction were also investigated. The results showed that the use of 70% ethanol for 90 seconds and 1.5% sodium hypochlorite for 8 minutes reduced the contamination and increased the viability of the samples. The highest callus formation was observed in half-strength MS medium contained 2,4-D (0.16 mg/L) and BAP (1.5 mg/L) or 0.08 mg /L of 2,4-D and 2 mg/L of BAP as well. Also, the highest rate of induction of somatic embryogenesis was observed using leaf explants in half-strength MS medium contained 2 mg/L 2,4-D and 0.3 mg/L BAP. Hormonal treatment including 0.2 mg/L BAP and 0.1 mg/L NAA in the modified MS medium could improve the direct regeneration of explants. Furthermore, the highest shoot length and number of shoots were obtained from hormonal treatment contained 0.1 mg/L NAA and 0.2 mg/L BAP and the highest rooting was observed in 0.6 mg/L 2,4-D and 1.5 mg/L BAP. The results of the current study revealed that sucrose concentration at 2% concentration has a significant effect on the percentage of embryogenesis and callus formation and using of ammonium nitrate at 200 mg/L concentration in half-strength MS medium can increase the anthurium regeneration. Also, treatment of 1 mg/L NAA and 0.5 mg/L BAP could significantly increase the root length. In this study, it was determined that the age of the explant, and the size of the explant have a significant effect on the rate of induction of callus formation, embryogenesis and direct regeneration. Younger explants were much faster and mor able to induce callus, embryo, and direct organogenesis, and also larger explants had more direct and indirect regeneration. The obtained results can be used in the promotion of methods related to anthurium micropropagation.